Skip to Main content Skip to Navigation
Preprints, Working Papers, ...

Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis

Abstract : Background The increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific community in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. Cathelicidin-related antimicrobial peptides (CRAMPs) from snake venoms have been studied as a model for the design of new antimicrobial pharmaceuticals against bacterial infections. Results In this study we present an 11 amino acid-long peptide, named pseudonajide, which is derived from a Pseudonaja textilis venom peptide and has antimicrobial and antibiofilm activity against Staphylococcus epidermidis . Pseudonajide was selected based on the sequence alignments of various snake venom peptides that displayed activity against bacteria. Antibiofilm activity assays with pseudonajide concentrations ranging from 3.12 to 100 µM showed that the lowest concentration to inhibit biofilm formation was 25 µM. Microscopy analysis demonstrated that pseudonajide interacts with the bacterial cell envelope, disrupting the cell walls and membranes, leading to morphological defects in prokaryotes. Conclusions Our results suggest that pseudonajide’s positives charges interact with negatively charged cell wall components of S. epidermidis, leading to cell damage and inhibiting biofilm formation.
Document type :
Preprints, Working Papers, ...
Complete list of metadatas

Cited literature [57 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02914418
Contributor : Sylvie Nonin-Lecomte <>
Submitted on : Tuesday, August 11, 2020 - 6:08:23 PM
Last modification on : Thursday, August 13, 2020 - 3:32:47 AM

File

2020-preprint-pseudonajide.pdf
Files produced by the author(s)

Identifiers

Citation

Rafael Schneider, Muriel Primon-Barros, Rafael Gomes Von Borowski, Sophie Chat, Sylvie Nonin-Lecomte, et al.. Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis. 2020. ⟨hal-02914418⟩

Share

Metrics

Record views

81

Files downloads

38